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EMBOSS: findkm |
%findkm Calculates Km and Vmax for an enzyme reaction. Enter name of file containing data: enztest.dat Display on device [xwin]:
Mandatory qualifiers: [-infile] infile Enter name of file containing data [-outfile] outfile Output file name -graphlb xygraph Graph type Optional qualifiers: (none) Advanced qualifiers: -[no]plot bool S/V vs S General qualifiers: -help bool report command line options. More information on associated and general qualifiers can be found with -help -verbose |
Mandatory qualifiers | Allowed values | Default | |
---|---|---|---|
[-infile] (Parameter 1) |
Enter name of file containing data | Input file | Required |
[-outfile] (Parameter 2) |
Output file name | Output file | <sequence>.findkm |
-graphlb | Graph type | EMBOSS has a list of known devices, including postscript, ps, hpgl, hp7470, hp7580, meta, colourps, cps, xwindows, x11, tektronics, tekt, tek4107t, tek, none, null, text, data, xterm, png | EMBOSS_GRAPHICS value, or x11 |
Optional qualifiers | Allowed values | Default | |
(none) | |||
Advanced qualifiers | Allowed values | Default | |
-[no]plot | S/V vs S | Yes/No | Yes |
The data for the example run are shown below:
0.10 2 0.20 4 0.50 9 1.00 17 2.00 29 5.00 50 10.00 67 20.00 80 50.00 91 100.00 95
---Hanes Woolf Plot Calculations--- Slope of best fit line is a = 0.01 Coefficient in Eqn of line y = ma +b is b = 0.05 Where line cuts x axis = (-0.20, 0) Where line cuts y axis = (0, 0.05) Limit-point of graph for plot = (103.00, 1.08) Vmax = 99.70, Km = 4.894612Click here for the graphical output
Program name | Description |
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This application was modified for inclusion in EMBOSS by David Martin (david.martin@biotek.uio.no) at the Norwegian EMBnet node.
Modified May 2000