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EMBOSS: pepnet |
It is therefore easy to see patterns of amphipathicity that you may wish to investigate in more detail by using displays such as pepwheel.
You can specify which residues to mark up in squares, diamonds and octagons.
% pepnet -sask Input sequence: sw:gcn4_yeast Begin at position [start]: 253 End at position [end]: 274 Graph type [x11]:
Mandatory qualifiers (* if not always prompted): [-sequence] sequence Sequence USA * -graph graph Graph type Optional qualifiers (* if not always prompted): * -squares string By default the aliphatic residues ILVM are marked with squares. * -diamonds string By default the residues DENQST are marked with diamonds. * -octags string By default the positively charged residues HKR are marked with octagons. Advanced qualifiers: -amphipathic bool If this is true then the residues ACFGILMVWY are marked as squares and all other residues are unmarked. This overrides any other markup that you may have specified using the qualifiers '-squares', '-diamonds' and '-octags'. -data bool Output the data to a file instead of plotting it |
Mandatory qualifiers | Allowed values | Default | |
---|---|---|---|
[-sequence] (Parameter 1) |
Sequence USA | Readable sequence | Required |
-graph | Graph type | EMBOSS has a list of known devices, including postscript, ps, hpgl, hp7470, hp7580, meta, colourps, cps, xwindows, x11, tektronics, tekt, tek4107t, tek, none, null, text, data, xterm | EMBOSS_GRAPHICS value, or x11 |
Optional qualifiers | Allowed values | Default | |
-squares | By default the aliphatic residues ILVM are marked with squares. | Any string is accepted | ILVM |
-diamonds | By default the residues DENQST are marked with diamonds. | Any string is accepted | DENQST |
-octags | By default the positively charged residues HKR are marked with octagons. | Any string is accepted | HKR |
Advanced qualifiers | Allowed values | Default | |
-amphipathic | If this is true then the residues ACFGILMVWY are marked as squares and all other residues are unmarked. This overrides any other markup that you may have specified using the qualifiers '-squares', '-diamonds' and '-octags'. | Yes/No | No |
-data | Output the data to a file instead of plotting it | Yes/No | No |
Program name | Description |
---|---|
checktrans | Reports STOP codons and ORF statistics of a protein sequence |
digest | Protein proteolytic enzyme or reagent cleavage digest |
garnier | Predicts protein secondary structure |
helixturnhelix | Report nucleic acid binding motifs |
iep | Calculates the isoelectric point of a protein |
octanol | Displays protein hydropathy |
pepcoil | Predicts coiled coil regions |
pepinfo | Plots simple amino acid properties in parallel |
pepstats | Protein statistics |
pepwheel | Shows protein sequences as helices |
pepwindow | Displays protein hydropathy |
pepwindowall | Displays protein hydropathy of a set of sequences |
sigcleave | Reports protein signal cleavage sites |